CPSA 2010
From Data to Biology:
Analytical Measurements to Drive Pharmaceutical R&D
October 18 - 21, 2010
Langhorne, PA
Short Courses
Monday, October 18, 2010
Sheraton Bucks County Hotel
Langhorne, Pennsylvania
The CPSA Short Courses focus on specialized training. These workshop-style events provide a unique opportunity to learn about current industry practices, emerging applications, and innovative analytical technologies. CPSA Short Course Instructors create a truly dynamic learning environment as key fundamentals are reviewed and first-hand experiences and practical case studies are openly discussed.
Method Development for LC/MS: Traditional Approaches and Emerging Trends
This practical course on HPLC method development for LC/MS is a tutorial on LC/MS/MS in the bioanalytical laboratory. As a conduit for learning, a major portion of the course is a step-wise method development tutorial for how to develop LC/MS/MS methods based on compound structure. The course discusses the importance of HPLC when interfaced with mass spectrometry including the HPLC and SPE separation of undetected matrix components (cause for “ion suppression”), isomers and labile metabolites. Much of the course is geared towards development of HPLC methods for LC/MS for use in regulated quantitative bioanalysis. A significant portion of the short course will be devoted to development and validation approaches of dried blood spot analysis methods in the LC/MS/MS bioanalytical laboratory.
Instructors:
Roger Hayes, Schering-Plough Research Institute
Shane Needham, Alturas Analytics
LC and MS Strategies for Method Development, Profiling and High Throughput Workflow Solutions with Emphasis on Measuring Drug Compounds and Endogenous Markers in Drug Discovery
Integrated LC and MS approaches for high throughput analysis have become standard practice in a pre-clinical drug discovery setting. Successful support strategies often provide a blend of high throughput methodologies with appropriate resources (automation tools and personnel). This course will target the bread and butter functions of using LC for drug discovery support. The focus will be on the use of MS for detection, but a range of other detectors also will be considered in the context of meeting specific chemical and biological needs in the preclinical setting. Real-world experiences with method development and analytical support will be thoroughly explored with emphasis on high probability of success and timely delivery of results. Many examples will feature ultra high throughput sample processing, fast LC (theory & practice), generic gradient RP method strategies, building in robustness into LC/MS set ups, Open-Access (self service) analyses, and rapid profiling of compounds for physico-chemical / metabolic parameters as well as markers indicating efficacy. Additional examples will be discussed that examine the challenges and approaches toward achieving very high sensitivity, performing difficult bioanalytical separations (including chiral), and using IT as well as automation based solutions in streamlining workflows.
Instructors:
Mark Hayward, Lundbeck Research USA
Ken Lewis, OpAns
Is Poor Bioavailability in Early drug Discovery a Problem and If So, How Can We Solve It?
Poor oral bioavailability is one of the leading causes of compound failure in preclinical and clinical development. Compounds with poor oral bioavailability have low plasma exposure and tend to demonstrate high inter-individual variability, which can limit their therapeutic application. Poor oral bioavailability in preclinical species does not necessarily translate into poor human oral bioavailability. This practical/hands-on course is designed to increase participants knowledge of:
- The parameters that determine drug oral bioavailability
- The factors that lead to species differences in oral bioavailability and how to deal with them
- Approaches for optimizing the physicochemical parameters that influence drug solubility/permeability and metabolism
- The workshop will also include a hands-on session that aims at improving your ability to apply these strategies to medicinal chemistry for hit selection, lead optimization and development candidate selection
Targeted Audience: Medicinal Chemists, Discovery Biologists, ADME Scientists
Instructor:
Ayman El-Kattan, Pfizer
Analysis of Peptides and Proteins: Sample Preparation to Identification to Quantitation
This year’s CPSA program highlights a key trend within the pharmaceutical
industry: The rapidly growing demand for the quantitative analysis of proteins and
peptides by LC-MS/MS. From the opening plenary lecture on Tuesday morning,
the Wednesday biologics session, through to the Thursday biomarkers session,
this short course will help conference participants deeply appreciate specific
challenges associated with highly successful outcomes in LC-MS based protein
analysis. In line with these sessions at the conference program, this preprogram
short course will provide an indepth view of the practical aspects involved in
protein/peptide analysis by LC-MS/MS.
The use of nanospray enabled MS has developed from a qualitative tool
for (global) proteomics to a quantitative method suitable for peptide/protein
biomarker validation. Key to success has been the combination of highly specific
sample preparation methods, high sensitivity nanospray ionization, and high
performance tandem mass spectrometry. Critical parameters involved in robust
sample preparation, nanobore LC and nanospray, sensitive & selective MS
detection along with their analytical benefits will be emphasized. The transition of
the traditionally qualitative nLC-MS/MS technology to those suitable for absolute
quantification will be discussed. Real world examples from the literature will be
presented. The target audience includes analysts that have been engaged in
qualitative proteomics wishing to transition to quantitative methods or analysts
that have specialized in small molecule quantification wishing to transition to
peptide/protein quantification.
Instructors:
Nalini Sadagopan, Agilent Technologies
Gary A. Valaskovic, New Objective
Chuck Witkowski, Protein Discovery
Fundamentals of Structural Characterization by NMR
The "Fundamentals of Structural Characterization by NMR" short-course will cover the essentials of both 1H and heteronuclear NMR as applicable to structural problems in the organic chemistry environment. This will be a hands-on, focused discussion. We will begin with a quick discussion of the fundamental aspects of the NMR experiment that have a direct impact on the final spectra and proceed through the basics of 1D NMR interpretation. Subsequent sections will focus on a particular type of experiment (e.g., Nuclear Overhauser Experiments, proton-proton coupling experiments, proton-carbon coupling experiments, etc.). The information available from each type of experiment will be discussed using examples and case studies. Each segment will build on the previous material and grow into a complete discussion of comprehensive assignment strategies for small molecule NMR problems.
Instructor:
David J. Russell, Varian
Understanding Autoradiography in Drug Discovery and Development
This course is intended for scientists and other interested people in the pharmaceutical industry who would like to understand how Quantitative Whole-Body Autoradiography (QWBA) and Micro-Autoradiography (MARG) are used in the discovery and development of small and large molecule pharmaceuticals and Biotech Entities. The course will present a detailed technical description of the techniques and instrumentation used to perform QWBA and MARG studies. It will provide researchers with more confidence and understanding about the data obtained and how the techniques can be applied to support their research in pre-clinical and clinical drug safety, pharmacology, drug metabolism, pharmacokinetics, and other areas of pharmaceutical research. Discussions on: history; method development; phosphor imaging; image analysis & quantitation; limitations, Validation/Regulatory concerns, rat anatomy in the sagital plane, applications (including Human Dosimetry Predictions), technology and principles.
Instructor:
Eric Solon, QPS, LLC
HPLC Method Development for LC
This course will focus on the practical solutions for the tough chromatographic separation and sample clean-up of polar compounds, in particular the small molecules and some oligomers of biological compounds. The course will discuss the mechanism and practical respects of a number of phases used in the HPLC and SPE for polar compounds, including the reverse phases, normal phases, ion-exchange phases and HILIC phases. Based on the understanding of the mechanism and properties of the phases, one may further learn from the course how to select a right HPLC column, a right sample preparation method, as well as how to develop systematically a method for both sample clean-up and HPLC separation in LC or LC-MS applications. A number of new sample preparation methods and new column chemistries will be introduced for the improved separation of polar compounds. A mix-phase strategy will be presented as a tool for a quick separation optimization of complex mixtures, and a tool for retaining polar ionic compounds by HPLC while compatible for LC-MS. The advantages and disadvantages of different SPE methods will also be discussed in term of the convenience, throughputs, effectiveness and robustness, especially for polar compounds.
Instructor:
Jerry Wang, Agela Technologies