Final ProgramCPSA 2011
Science and Technology Coming Together to Make a Difference
October 3 - 6, 2011
Bucks County Sheraton Hotel
Langhorne, PA
Poster Abstract #16
Multiplexing SIMs on a Novel Benchtop Orbitrap MS with a Quadrupole Mass Filter to Increase Sensitivity for Peptide Quantitation
Thermo Fisher Scientific, San Jose, California, USA
In bioanalysis, triple quadrupole MS systems have been predominantly used for peptide quantitation. Although triple quadrupole systems generally offer the most sensitive and robust quantitation, the poor fragmentation and multiple charge states of large peptides can result in weak selected reaction monitoring (SRM) signals. In this investigation, we demonstrate the accurate and precise quantification of peptides in a biological matrix suing a new approach that utilizes multiplexed SIMs capability provided on a novel bench top quadrupole-Orbitrap mass spectrometer. Multiplexed SIMs refers to multiple fills of the instrument’s C-trap with quadrupole-filtered ions, prior to a Orbitrap mass analyzer scan. We employed a ten-minute LC method at 150 µL/min.-1 Mass spectrometry analysis was performed using a quadrupole-Orbitrap LC-MS/MS systems equipped with a heated electrospray ionization (HESI) source. A generic method development approach was used to analyze Insulin, Exendin-4 and GLP-1 peptides, between 10 pg.mL-1 and 10 µg.mL,-1 with good linearity and reproducibility.