Final ProgramCPSA 2011
Science and Technology Coming Together to Make a Difference
October 3 - 6, 2011
Bucks County Sheraton Hotel
Langhorne, PA
Poster Abstract #17
Analysis of a Therapeutic Antibody and a Biomarker in Parallel Utilizing Immunocapture and LC-MS/MS
GlaxoSmithKline Pharmaceuticals Bioanalytical Science and Toxicokinetics, PTS DMPK King of Prussia, PA USA
A method was developed to measure a therapeutic antibody and its corresponding antigen in a single LC-MS/MS method. Immunoassay was not viable as there were selectivity and reagent availability issues. The method was needed for support of a safety assessment study planned using transgenic mice. Because of limited sample volume a dual LC-MS/MS assay measuring both the therapeutic and SAP (PD biomarker) needed to be developed. The poster will show method development and validation work done to develop both antibody and antigen methods for safety assessment support.